Source of variation detected in ribotyping patterns of Haemophilus influenzae: comparison of traditional ribotyping, PCR-ribotyping and rDNA restriction analysis
نویسندگان
چکیده
منابع مشابه
PCR-based ribotyping of Staphylococcus aureus.
Genotyping techniques are valuable tools for the epidemiologic study of Staphylococcus aureus infections in the hospital setting. Pulsed-field gel electrophoresis (PFGE) is the current method of choice for S. aureus strain typing. However, the method is laborious and requires expensive equipment. In the present study, we evaluated the natural polymorphism of the genomic 16S-23S rRNA region for ...
متن کاملRibotyping, biotyping and capsular typing of Haemophilus influenzae strains isolated from patients in Campinas, southeast Brazil.
Forty-five Haemophilus influenzae strains isolated from patients were characterized based on biochemical characteristics. Their capsular types were determined by polymerase chain reaction (PCR); they were compared, using two molecular methods [ribotyping with a specific DNA probe amplified from the 16S rDNA region from H. influenzae and through restriction fragment length polymorphism (RLFP) of...
متن کاملSerotyping and ribotyping of Salmonella using restriction enzyme PvuII.
The subtyping and identification of bacterial pathogens throughout food processing and production chains is useful to the new hazard analysis critical control point-based food safety plans. Traditional manual serotyping remains the primary means of subtyping Salmonella isolates. Molecular biology techniques, however, offer the promise of more rapid and sensitive subtyping of Salmonella. This st...
متن کاملCharacterization of Bacteria by Ribotyping
Ribotyping, a molecular method based on the analysis of restriction fragment length polymorphisms (RFLPs) of ribosomal RNA genes, has been widely adopted to distinguish a variety of bacteria (1-5). For the past five years ATCC has been using the RiboPrinter® Microbial Characterization system (Qualicon Inc., Wilmington, DE) as a primary method for characterizing strains during the quality contro...
متن کاملPcr-ribotyping of Bovine and Human Methicillin- Resistant Staphylococcus Aureus
The present study aimed at the assessment of genetic relatedness among the methicillin-resistant Staphylococcus aureus (MRSA) isolates of bovine and human origin. A set of twenty-four MRSA isolates obtained from cattle (11), buffaloes (10) and their handlers (3) were subjected to PCR-ribotyping using primers specific for 16S-23S ribosomal spacer region. Polymorphism was observed with 23 ribotyp...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 1997
ISSN: 0022-2615,1473-5644
DOI: 10.1099/00222615-46-9-763